R-Factor Transfer in Selenite and Tetrathionate Broths

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R-factor transfer in selenite and tetrathionate broths.

After overnight incubation of R(+)Escherichia coli with R(-)Salmonella typhimurium in selenite and tetrathionate with Brilliant Green (TBG) broths, R-factor transfer was demonstrated in 10 of 12 experiments. R-factor transfer in these enrichment broths occurred at a markedly reduced frequency in comparison to that in Trypticase soy broth, apparently due to an adverse effect either on the viabil...

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Anaerobic R factor transfer in Escherichia coli.

R factor mediated transfer of antibiotic resistance between Enterobacteriaceae has been reported to occur in the mammalian gastrointestinal tract (Kasuya, I 964; Reed, Sieckmann & Georgi, 1969; Guinie, 1970; Wiedemann, Knothe & Doll, 1970) where conditions are mainly anaerobic as demonstrated by the abundance of obligate anaerobic bacteria such as Bacteroides species (Moore, Cat0 & Holdeman, 19...

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Isolation of Salmonellae from food samples. IV. Comparison of methods of enrichment.

A comparison of various methods of enhancing frequency of Salmonella isolations revealed that inoculation of a second enrichment broth, with culture from the first, was no improvement over the single direct enrichment method. It was inferior to centrifugation. Selenite was observed to produce more positive isolations at 48 hr than at 24. No change occurred in tetrathionate. Reconstitution of dr...

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Seven-thousand-and-thirty-six specimens of faeces were examined for Salmonellae using three enrichment culture media in parallel.Rappaport's magnesium chloride malachite green broth medium proved superior to selenite F and tetrathionate broths in the recovery of a wide variety of Salmonella serotypes. However, it was inferior to selenite broth in the recovery of Salmonella typhi.A higher recove...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1971

ISSN: 0003-6919

DOI: 10.1128/aem.21.4.685-687.1971